The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR items were digested and cloned right into a pET21a+ vector employing NdeI and XhoI restriction enzymes. A point mutation was released by the quick change https://conanw110lmt3.blog-ezine.com/profile